Prof. Dr. Reinhard Rachel
Centre for EM - @ Institute for Anatomy
D-93053 Regensburg - Germany
Tel: +49-(0)941-943-2837 oder -1720
Office: VKL 3.1.29
- Vereinigung für Allgemeine und Angewandte Mikrobiologie
ISE - International Society of Extremophiles
Deutsche Gesellschaft für Elektronenmikroskopie (Vorstandsmitglied)
European Society for Microscopy
Cell-cell interaction among Archaea: Ignicoccus and Nanoarchaeum
So far, we were able to show that all cells of the genus Ignicoccus are cocci, with an unusual and unique ultrastructure (Rachel et al 2002; Näther und Rachel 2004; Paper et al. 2007). The tightly packed cytoplasm is delineated by a highly dynamic cytoplasmic membrane, forming round or elongated vesicles into the periplasm. The periplasm itself is variable in width, 20 to 400 nm in diameter, and its relative volume is – compared with other Archaea – unusually large, much larger than the cytoplasm. The outer boundary of the periplasm is an outer membrane, the first and still only one observed among the Archaea. It is composed of phytanyl ether lipids and pore-forming proteins. The major, dominating protein Imp1227 was recently characterized in detail (Burghardt et al. 2007). The mass of its monomer is only 6.2 kDa, forming stable oligomers (they do not dissociate even at 100°C in 2% SDS). These complexes with a diameter of 7 nm are tightly packed in the outer membrane and possess a central pore, 2 nm in diameter. Current investigations aim to purify it to homogeneity, to characterize its detailed structure by crystallography (in collaboration with the Biocentre in Basel, Prof. Dr. Tilman Schirmer), and to study its function, by analyzing the specificity of the pore, in artificial lipid membranes. In addition, we want to understand the function of this protein and of 'flagella'-like appendages (see project 2) for the docking of Nanoarchaeum equitans to Ignicoccus hospitalis.
Supported by a grant of the DFG (SPP 1112)
Cell appendages of species of the genus Ignicoccus
Supported by a grant of the DFG
Coworker: Carolin Meyer, Nadine Wasserburger, Prof.
Dr. Reinhard Wirth, Reinhard Rachel
Directed dissolution of Pyrit using acidophilic Microorganisms: Cell-surface interactions
In this project, we employ fluorescence light microscopy and scanning and transmission electron microscopy, in order to study the etching of Pyrite by four different microorganisms, two Bacteria and two Archaea. The major factors investigated are: the influence of time of incubation, the surface of the pyrite crystal, on which the microorganisms prefer to adhere; and the type of microbial surface structures involved.
Supported by a grant of the DFG
Klingl, Cordula Neuner, Harald Huber, Michael
Thomm, Reinhard Rachel; in collaboration with Prof.
Dr. G. Schmalz, University Hospital Regensburg, Dentistry, and
Prof. Dr. W.
Depmeier and Katja Etzel, University of Kiel, Institute for Geoscience.
Ultrastructure of Mouse Kidney
The major aim of this project is the investigation of kidney ultrastructure of mice in which selected genes have been "knocked-out". The preparation involves ultrathin sections of kidney samples prepared by standard embedding techniques following perfusion fixation; in addition, micro-biopsies are prepared by high-pressure freezing, freeze-substiution, resin-embedding and ultrathin sectioning. In particular, we want to study the ultrastructural changes at the glomerulum and at proximal and distal tubuli.
Supported by a grant of the DFG (SFB 699, Projekt Z2)
Principal investigators: Prof. Dr. R. Witzgall, Prof. Dr. E. Tamm,
Prof. Dr. A. Kurtz, Prof. Dr. R. Warth
Instrumentation and Methods:
The Faculty Centre for EM also has a preparation lab, including:
of Prof. Dr. Reinhard Wirth, Microbiology
Lab of Dr. Harald Huber, Microbiology
Inst. for Anatomy, Prof. Dr. R. Witzgall and Prof. Dr. E. Tamm
Inst. for Biochemistry, Prof. Dr. H. Tschochner
Inst. for Zoology, Prof. Dr. S. Schneuwly
Schwerpunktpraktikum Organism. Mikrobiologie im Sommersemester