Prof. Dr. M. Thomm
Coworkers: Christoph Reich
Interactions of archaeal and eukaryotic subunits
On the other hand, we have the yeast system, which is a well developed genetic system and we can use the information provided by the solved crystal structure of RNAP II (Fig. 2). In the basic archaeal system we can take advantage of the in vitro reconstituted RNAP of Pyrococcus which was established in our group.
The main questions in this project are:
1. Do archaeal RNAP subunits work in the yeast?
In this attempt
we perform shuffle experiments to find out if archaeal subunits or fusion-proteins
consisting of eukaryal and archaeal parts can complement the Saccharomyces
2. Do eukaryotic subunits work in the archaeal system?
this approach we purify eukaryotic subunits and incorporate them in
a reconstituted Pyrococcus RNAP. Additionally we investigate
RNAPs lacking a certain subunit.
our results so far it seems probable that the archaeal RNAP really is
a basal system and a good model for the eukaryotic transcription.