Hauska´s Lab Home Page

[Picture of Mr. Hauska]

Adress:


Prof. Dr. G. Hauska
Universität Regensburg
Lehrstuhl für Zellbiologie und Pflanzenphysiologie
Universitätsstraße 31
93053 Regensburg, Germany

Telefone: (0941) 943 3031
Telefax: (0941) 943 3352
e-mail: guenther.hauska@biologie.uni-regensburg.de

Career:

1967 PhD in Chemistry with O.Hoffmann-Ostenhof, University of Vienna

1968-1970 Postdoc with R.E.McCarty and E.Racker, Cornell University

1971-1976 Research assistant with A.Trebst, Ruhr-Universität Bochum

1973 Habilitation in biochemistry, Ruhr-Universität Bochum

since 1976 Professor at the Institut für Botanik, Universität Regensburg

1989 - 1990 Sabbatical with N.Nelson, Roche Institute of Molecular Biology, Nutley, NJ USA 


Research Interest:

Principles of Energy Conservation in the Electron Transport Chains of Photosynthesis and Respiration

Further research interests: see also here! (Non-official homepage!)
 
 

Research contributions in the past /collaborations:

Current lines of research:

At present we are concerned with the following projects:
    1. We are attempting to crystallize the homodomeric P840-RC (collaboration with Petra Fromme / Berlin, Nathan Nelson / Tel Aviv, Georgios Tsiotis / Iraklion, Greece).
    2. We are pursuing to elucidate bc-complexes of phylogenetic old bacteria like the genera Aquifex and Chloroflexus to find still further variants in addition to Chlorobium(together with Michael Schütz and Wolfgang Nitschke / Marseille).
    3. We have succeeded in purifying the sulfide-quinone oxidoreductase from Rhodobacter capsulatus to homogeneity, and have cloned, sequenced and expressed its gene. We are now investigating its catalytic mechanism and its regulation (collaboration with E.Padan/Jerusalem and Y.Shahak/Bet Dagan funded by the DFG), as well as its possible use in microsensing of sulfide (Otto Wolfbeis/Department of Analytical Chemistry, Chemo- and Biosensors).
    4. We have become interested in the redox sensing role of the b6f-complex (ref. 14), and presently investigate its role in heterocyst formation of filamentous cyanobacteria (together with Iris Maldener).

Selected References

Original Papers:
  1. Hauska G, McCarty R E, Berzborn R J and Racker E (1971), Partial resolution and reconstitution of the enzymes catalyzing photophosphorylation VII. The function of plastocyanin and its reaction with a specific antibody, J. Biol. Chem. 246, 3524 - 3531
  2. Prince R C, Baccarini-Melandri A, Hauska G, Melandri A B and Crofts A R (1975), Asymmetry of an energy transducing membrane; the location of cytochrome c2 in Rhodopseudomonas sphaeroides and Rhodopseudomonas capsulata., Biochim. Biophys. Acta 387, 212 - 227
  3. Hauska G, Trebst A and Reimer S (1974), Native and artificial energy-conserving sites in cyclic photophosphorylation systems with photosystem I., Biochim. Biophys. Acta 357, 1 - 13
  4. Futami A, Hurt E and Hauska G (1979), Vectorial redox reactions of physiological quinones I. Requirement of a minimum of length of the isoprenoid side chain, Biochim. Biophys. Acta 547, 583 - 596
  5. Hauska G, Orlich G, Samoray D and Nelson N (1980), Reconstitution of photosynthetic energy conservation - photophosphorylation in liposomes containing P700-reaction centers and DCCD-sensitive ATPase, Eur. J. Biochem. 111, 535 - 543
  6. Hurt E C and Hauska G (1981), A Cytochrome b6f Complex of Five Polypeptides with Plastoquinol-Plastocyanin Oxidoreductase Activity from Spinach Chloroplasts,. Eur. J. Biochem. 117, 591 - 599
  7. Krinner M, Hauska G, Hurt E and Lockau W (1982), A Cytochrome b6f Complex with Plastoquinol-Plastocyanin Oxidoreductase Activity from Anabaena variabilis, Biochim, Biophys. Acta 681, 110 - 117
  8. Gabellini N, Bowyer J R, Hurt E, Melandri B A and Hauska G (1982), A Cytochrome bc1 Complex with Ubiquinol-Cytochrome c2 Oxidoreductase Activity from Rhodopseudomonas sphaeroides GA, Eur. J. Biochem. 126, 105 - 111
  9. Hurt E C and Hauska G (1983), Cytochrome b6 from Isolated Cytochrome b6f Complexes - Evidence for two Spectral Forms with different Midpoint Potentials, FEBS Lett. 153, 413 - 419
  10. Hurt E C, Gabellini N, Shahak Y, Lockau W and Hauska G (1983), Extra Proton Translocation and Membrane Potential Generation - Universal Properties
  11. of Cytochrome bc1/b6f Complexes Reconstituted into Liposomes, Arch. Biochem.Biophys. 225, 879 - 885
  12. Hurt E and Hauska G (1984), Purification of Membrane-Bound Cytochromes and a Photoactive P840 Protein Complex of the Green Sulfur Bacterium Chlorobium limicola f. thiosulfatophilum, FEBS Lett. 168, 149 - 154
  13. Nitschke W, Hauska G and Crofts AR (1988), Fast Electron Transfer from Low- to High-Potential Cytochrome b6 in Isolated Cytochrome b6f-Complex, FEBS Letters 232, 204-208
  14. Gal A, Hauska G, Herrmann R and Ohad I (1990), Interaction between Licht Harvesting Chlorophyll a/b-Protein (LHCII)-Kinase and Cytochrome b6f-Complex: In vitro Control of Kinase Activity, J.Biol.Chem. 265, 19742-19749
  15. Büttner M, Xie D-L, Nelson H, Pinther W, Hauska G and Nelson N (1992), Photosynthetic Reaction Center Genes in Green Sulfur Bacteria and in Photosystem 1 are related, Proc.Natl.Acad.Sci.USA 89, 8135-8139
  16. Shahak Y, Klughammer C, Schreiber U, Padan E, Herrmann I and Hauska G (1994), Sulfide-Quinone and Sulfide-Cytochrome Reduction in Rhodobacter capsulatus, Photosynth.Res. 39, 175-181
  17. Schütz M, Zirngibl S, le Coutre J, Büttner M, Xie D-L, Nelson N, Deutzmann N and Hauska G (1994), A Transcription Unit for the Rieske FeS-Protein and Cytochrome b in Chlorobium limicola Combines Characteristics of the bc1/b6f-Types, Photosynth.Res. 39, 163-174
  18. Klughammer C, Hager C, Padan E, Schütz M, Schreiber U, Shahak Y und Hauska G (1995), Reduction of cytochromes with menaquinol and sulfide in membranes from green sulfur bacteria, Photosynth.Res. 43, 27-34
  19. Hager-Braun C, Xie D-L, Jarosch U, Herold E, Büttner M, Zimmermann R, Deutzmann R, Hauska G und Nelson N (1995), Stable photobleaching of P840 in Chlorobium reaction center preparations: Presence of the 42-kDa bacteriochlorophyll a protein and a 17-kDa polypeptide, Biochemistry 34, 9617-9624
  20. Frankenberg N, Hager-Braun C, Feiler U, Fuhrmann M, Rogl H, Schneebauer N, Nelson N and Hauska G (1996), P840-reaction centers from Chlorobium tepidum - quinone analysis and functional reconstitution into lipid vesicles, Photochem.Photobiol., 64,14-19
  21. Schütz M, Shahak Y, Padan E und Hauska G (1997), The Sulfide-Quinone-Reductase from Rhodobacter capsulatus: Purification, Sequencing and Expression, JBC., 272, 9890-9894
  22. Hager-Braun C, Jarosch U, Hauska G, Nitschke W and Riedel A (1997), The terminal electron acceptors of green sulfur bacterial reaction centre in EPR. Photosynth. Res., 51, 127-136
  23. Tsiotis G, Hager-Braun C, Wolpensinger B, Engel A and Hauska G (1997) Structural analysis of the photosynthetic reaction center from the green sulfur bacterium Chlorobium tepidum. Biochim. Biophys.Acta 1322, 163-172
  24. Schütz M, Griesbeck C, Bronstein M, Maldener I, Shahak Y, Padan E and Hauska G (1998) Sulfide-Quinone Reductase (SQR) of Rhodobacter capsulatus: expression, induction and inactivation. In: Garab G (ed.) International Congress on Photosynthesis (Budapest, 1998)
  25. Schütz M, Klughammer C, Griesbeck C, Quentmeier A, Schreiber U, Friedrich CG und Hauska G (1998) Sulfide-quinone reductase (SQR) activity in membranes of the chemotrophic bacterium Paracoccus denitrificans GB17. Arch. Microbiol. 170, 353-360
  26. Griesbeck C, Hager-Braun C, Rogl H und Hauska G (1998) Quantitation of P840 reaction center preparations from Chlorobium tepidum: chlorophylls and FMO-protein. Biochim. Biophys. Acta 1365, 285-293
  27. Rémigy H-W, Stahlberg, H, Fotiadis D, Mueller S, Wolpensinger B, Engel A, Hauska G and Tsiotis G(1999) The Reaction Center from the Green Sulfur Bacterium Chlorobium tepidum: a Structural Analysis by Scanning Transmission Electron Microscopy, J.Mol.Biol., 290: 851-858
  28. Schütz M, Maldener I, Griesbeck C and Hauska G (1999) Sulfide-Chinon Reductase from Rhodobacter capsulatus - Requirement for Growth, Periplasmic Localization and Extension of Gene Sequence Analysis,  J.Bacteriol.,181: 6516-6523
  29. Dirmeier R., Hauska G and Stetter K (1999) ATP Synthesis at 100°C by an ATPase purified from the hyperthermophilic archeon Pyrodictium abyssii, FEBS Letters 467, 101-104
  30. Nübel T, Klughammer C, Huber R, Hauska G and Schütz M (2000) Sulfide-quinone reductase in membranes of the hyperthermophilic bacterium Aquifex aeolicus (VF5), Arch. Microbiol. 173, 233-44

Reviews:

  1. Hauska G and Trebst A (1977), in: Current Topics in Bioenergetics (D. R. Sanadi ed.), Proton translocation in chloroplasts Vol. 6, 151 - 220, Acad. Pres
  2. Hauska G, Hurt E, Gabellini N and Lockau W (1983), Comparatative Aspects of Quinol-Cytochrome c/Plastocyanin Oxidoreductases, Biochim. Biophys. Acta 726, 97 - 133
  3. Hauska G, Nitschke W and Herrmann R (1988), Amino Acid Identities in the Three Redox Center Carrying Polypeptides of Cytochrome bc1/b6f-Complexes, J. Bioenerg. Biomemb. 20, 211-228
  4. Feiler U and Hauska G (1995), The Reaction Centre from Green Sulphur Bacteria, In: Advances in Photosynthesis, Anoxygenic Photosynthetic Bacteria (Blankenship RE, Madigan MT, Bauer CE eds.), pp 665-685, Kluwer Acad.Publ.
  5. Hauska G, Schütz M and Büttner M (1996), The cytochrome b6f complex - composition, structure and function, In: Advances in Photosynthesis, Oxygenic Photosynthesis (DR.Ort and Yokum CF eds.) Vol. 4, Kluwer Acad.Publ., pp. 377-398
  6. Shahak Y, Schütz M, Bronstein M, Griesbeck C, Hauska G und Padan E (1999) Sulfide-dependent anoxygenic photosynthesis in prokaryotes: Sulfide-quinone reductase (SQR) the initial step. In: Peschek GA, Löffelhardt W und Schmetterer G (eds.) Proceedings of the 9th International Symposium Phototrophic Procaryotes (Vienna, 1997). Plenum Press, New York, pp 217-228
  7. Griesbeck C, Hauska G and Schütz M (2000) Biological Sulfide Oxidation: Sulfide-Quinone Reductase (SQR), the Primary Reaction, Recent Research Developments in Microbiology 4, 179-203 (Download PDF-file)