Ribosome Maturation

Contact: Philipp Milkereit / Herbert Tschochner

Team Topic Collaborations Funding Publications

 

Team

Joachim Griesenbeck Principal investigator	Kristin Hegert Technical assistant	Thomas Hierlmeier PhD student
Jan Linnemann Technical assistant	Philipp Milkereit Principal investigator	Uli Ohmayer PhD student
Jorge Perez-Fernandez Postdoctoral researcher	Gisela Pöll Technical assistant	Herbert Tschochner Principal investigator

 

Topic

Ribosomes catalyze the translation of mRNA into proteins. Thereby, they have a key role in gene expression, cell growth and cell cycle progression. Ribosomes are large ribonucleo-protein complexes (RNP’s) consisting  of  ribosomal RNA (rRNA) and a large number (>50) of ribosomal proteins (r-proteins). Recent years brought detailed insights into the 3D-organisation of rRNA and r-proteins in mature ribosomes. In addition, it became evident that a significant proportion of the genome of an unicellular eukaryote (S. cerevisiae) codes for factors which are required for making ribosomes. Taking yeast  ribosome biogenesis as model system, we use a variety of in vivo and in vitro approaches to study the interrelationship between important  processes  common to  eukaryotic RNP maturation pathways, namely RNA synthesis, RNA processing, RNP assembly and intracellular transport of  RNP precursors.

 

Selected publications

A local role for the small ribosomal subunit primary binder rpS5 in final 18S rRNA processing in yeast.
Neueder A, Jakob S, Pöll G, Linnemann J, Deutzmann R, Tschochner H, Milkereit P. PLoS One. 2010 Apr 19;5(4):e10194.

Analysis of ribosome biogenesis factor-modules in yeast cells depleted from pre-ribosomes. Merl J, Jakob S, Ridinger K, Hierlmeier T, Deutzmann R, Milkereit P, Tschochner H. Nucleic Acids Res. 2010 May;38(9):3068-80. Epub 2010 Jan 25.

The Noc-domain containing C-terminus of Noc4p mediates both formation of the Noc4p-Nop14p submodule and its incorporation into the SSU processome. Kühn H, Hierlmeier T, Merl J, Jakob S, Aguissa-Touré AH, Milkereit P, Tschochner H. PLoS One. 2009 Dec 18;4(12):e8370.

rRNA maturation in yeast cells depleted of large ribosomal subunit proteins. Pöll G, Braun T, Jakovljevic J, Neueder A, Jakob S, Woolford JL Jr, Tschochner H, Milkereit P. PLoS One. 2009 Dec 11;4(12):e8249.

Analysis of the in vivo assembly pathway of eukaryotic 40S ribosomal proteins. Ferreira-Cerca S, Pöll G, Kühn H, Neueder A, Jakob S, Tschochner H, Milkereit P. Mol Cell. 2007 Nov 9;28(3):446-57.

Roles of eukaryotic ribosomal proteins in maturation and transport of pre-18S rRNA and ribosome function. Ferreira-Cerca S, Pöll G, Gleizes PE, Tschochner H, Milkereit P. Mol Cell. 2005 Oct 28;20(2):263-75.

The pre-ribosomal network.  Milkereit P, Kühn H, Gas N, Tschochner H. Nucleic Acids Res. 2003 Feb 1;31(3):799-804.

A Noc complex specifically involved in the formation and nuclear export of ribosomal 40 S subunits. Milkereit P, Strauss D, Bassler J, Gadal O, Kühn H, Schütz S, Gas N, Lechner J, Hurt E, Tschochner H. J Biol Chem. 2003 Feb 7;278(6):4072-81. Epub 2002 Nov 20.

Maturation and intranuclear transport of pre-ribosomes requires Noc proteins. Milkereit P, Gadal O, Podtelejnikov A, Trumtel S, Gas N, Petfalski E, Tollervey D, Mann M, Hurt E, Tschochner H. Cell. 2001 May 18;105(4):499-509.

Association of yeast RNA polymerase I with a nucleolar substructure active in rRNA synthesis and processing. Fath S, Milkereit P, Podtelejnikov AV, Bischler N, Schultz P, Bier M, Mann M, Tschochner H. J Cell Biol. 2000 May 1;149(3):575-90.

 

 

 

Collaborations

Jochen Bassler, BZH, Heidelberg, Germany.

Olivier Gadal, Laboratoire de Biologie Moléculaire Eucaryote, Université Paul Sabatier, Toulouse III, France.

Patrick Schultz, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.

John Woolford, Department of Biological Sciences, Carnegie Mellon, Pittsburgh, USA.

 

 

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Funding

 

• since July 2011

  DFG collaborative research Center SFB960 at the University of Regensburg: Ribosome formation: principles of RNP biogenesis and control of their function

  Project B1: Analyses of eukaryotic ribosome assembly

 

 

• April 2008 - June 2011

  DFG research group FOR1068 at the University of Regensburg: From Chromatin to the Ribosome: Regulation and Mechanisms of Ribosome-Biogenesis

  Projects: The function of heterodimer Noc-complexes and other non-ribosomal proteinmodules in the maturation of eukaryotic pre-ribosomes / Analysis of the in vivo functions of eukaryotic ribosomal proteins

   

   

• January 2008 - December 2010

  Projet International de Coopération Scientifique - PICS, together with the groups of Olivier Gadal and Pierre-Emmanuel Gleizes at the Université Paul Sabatier, Toulouse III, France.

  Project: Synthesis of the ribosomal subunits in the eukaryotic cells

   

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