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Contact: Herbert Tschochner
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| Jochen Gerber Postdoctoral researcher |
Joachim Griesenbeck Principal investigator |
Ribosomes, the cellular factories for protein production, are among the most intricate and complex ribonucleoprotein (RNP)-assemblies in the cell. Their biogenesis is tightly regulated and requires the coordinated activity of many cellular processes and can therefore be considered as a well-suited model system to study mechanisms of biogenesis of RNP complexes. Synthesis of the majority of ribosomal RNAs in eukaryotes is executed by a highly specialized RNA polymerase, Pol I. Our major interests are to understand mechanisms and regulation of the RNA polymerase I transcription machinery and to study how rRNA synthesis is coupled to rRNA maturation and assembly of pre-ribosomes.
Our goals are to reconstitute transcription initiation, elongation and termination from a minimal transcription system, to understand the roles of single subunits of the transcription machinery and to compare features of Pol I transcription to other nuclear RNA polymerases.
Reiter A, Steinbauer R, Philippi A, Gerber J, Tschochner H*, Milkereit P*, Griesenbeck J*. (2011) Reduction in Ribosomal Protein Synthesis Is Sufficient To Explain Major Effects on Ribosome Production after Short-Term TOR Inactivation in Saccharomyces cerevisiae. Mol Cell Biol. 2011 Feb;31(4):803-17 
*corresponding authors
Philippi, A., Steinbauer R., Reiter A., Fath S., Leger-Silvestre I., Milkereit P.*, Griesenbeck J. * and Tschochner H. * (2010) TOR-dependent reduction in the expression level of Rrn3p lowers the activity of the yeast RNA Pol I machinery, but does not account for the strong inhibition of rRNA production. Nucleic Acids Research, 2010, 38 (16), 5315-5326
*corresponding authors
Reich, C., Zeller, M., Milkereit, P., Hausner, W., Cramer, P., Tschochner, H. and Thomm, M. (2009) The archaeal RNA polymerase subunit P and the eukaryotic polymerase subunit Rpb12 are interchangeable in vivo and in vitro. Mol Microbiology 71, 989-1002
Clemente-Blanco, A., Mayán-Santos, M., Schneider, D.A., Machín, F., Jarmuz, A., Tschochner, H. and Aragón, L. (2009) Cdc14 inhibits transcription by RNA polymerase I during anaphase. Nature, 458, 219-222
Gerber, J., Reiter, A., Steinbauer, R., Jakob, S., Kuhn, C.-D., Cramer, P., Griesenbeck, J., Milkereit, P. and Tschochner, H. (2008) Site specific phosphorylation of yeast RNA polymerase I. Nucleic Acid Research, 36, 793-802
Kuhn, C.-D., Geiger, S., Baumli, S., Gartmann, M., Gerber, J., Jennebach, S., Mielke, T., Tschochner, H., Beckmann, R. and Cramer, P. (2007) Functional architecture of yeast RNA polymerase I. Cell, 131, 1260-1272
Fath, S., Kobor, M., Philippi, A., Greenblatt, M. and Tschochner, H. (2004) Dephosphorylation of RNA polymerase I by Fcp1p is required for efficient rRNA synthesis J. Biol. Chem. 279(24), 25251-9
Bier, M., Fath S. and Tschochner, H. (2004) The composition of the RNA polymerase I transcription machinery switches from initiation to elongation mode FEBS Lett. 564 (1-2), 41-46
Bischler, N., Brino, L., Carles, C., Riva, M., Tschochner, H., Mallouh, V. and Schultz, P. (2002) Localisation of the yeast RNA polymerase I-specific subunits. EMBO J. 21, 4136-4144
Iben, S., Tschochner, H., Bier, M., Hoogstraten, D., Hozak, P., Egly, J.-M. and Grummt, I. (2002) TFIIH plays an essential role in RNA polymerase I transcription Cell 109, 297-306
Fath, S., Milkereit, P., Peyroche, G., Riva, M., Carles, Ch. and Tschochner, H. (2001) Differential roles of phosphorylation in the formation of transcriptional active RNA polymerase I. Proc. Natl. Acad. Sci. USA 98, 14334-14339
Peyroche, G., Milkereit, M., Carles, C., Tschochner, H., Schultz, P., Sentenac, A. and Riva, M. (2000) The recruitment of RNA polymerase I on rDNA is mediated by the interaction of the A43 subunit with Rrn3. EMBO J. 19(20):5473-5482
Fath, S., Milkereit, P., Podtelejnikov, A., Bischler, N., Schultz, P., Mann, M. and Tschochner, H. (2000) Association of yeast RNA polymerase I with a nucleolar substructure active in rRNA synthesis and processing J. Cell Biol. 149, 575-589
Milkereit, P. and Tschochner H. (1998) A specialized form of RNA polymerase I, essential for initiation and growth dependent regulation of rRNA synthesis, is disrupted during transcription EMBO J. 17, 3692-3703
Bischler, N., Balavoine, F., Milkereit, P., Tschochner, H., Mioskowski, C. and Schultz, P. (1998) Specific interaction and two-dimensional crystallisation of histidine tagged yeast RNA polymerase I on Nickel-chelating lipids Biophys. J. 74, 1522-1532
Milkereit, P., Schultz, P. and Tschochner, H. (1997) Resolution of RNA polymerase I into dimers and monomers and their function in transcription Biol. Chem. 378, 1433-1443
Tschochner, H. and Milkereit, P. (1997) RNA polymerase I from S. cerevisiae depends on an additional factor to release terminated transcripts from the template. FEBS Lett. 410, 461-466
Tschochner, H. (1996) A novel RNA polymerase I-dependent RNase activity that shortens nascent transcripts from the 3´end. Proc. Natl. Acad. Sci. USA 93, 12914-12919
Wettach, J., Gohl, H.P., Tschochner, H. and Thomm, M. (1995) Functional interaction of yeast and human TATA-binding protein with an archael RNA polymerase and promoter. Proc. Natl. Acad. Sci. U.S.A. 92, 472-476
Christophe Carles, CEA Saclay, Gif-sur-Yvette, France.
Olivier Gadal, Laboratoire de Biologie Moléculaire Eucaryote, Université Paul Sabatier, Toulouse III, France.
Patrick Cramer, Gene Center Munich, Germany.
Thomas Moss, Centre de Recherche en Cancérologie, Université Laval, Quebec, Canada.
Patrick Schultz, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.
DFG collaborative research Center SFB960 at the University of Regensburg: Ribosome formation: principles of RNP biogenesis and control of their function
Project A1: Analyses of the RNA polymerase I machinery: specific transcriptional mechanisms and comparison with other RNA polymerases
DFG research group FOR1068 at the University of Regensburg: From Chromatin to the Ribosome: Regulation and Mechanisms of Ribosome-Biogenesis
Project: Formation of transcription-active RNA Polymerase I-complexes in yeast
Projet International de Coopération Scientifique - PICS, together with the groups of Olivier Gadal and Pierre-Emmanuel Gleizes at the Université Paul Sabatier, Toulouse III, France.
Project: Synthesis of the ribosomal subunits in the eukaryotic cells
